Checking Read Quality - FASTQC

FASTQC provides an overview of sequencing read quality.

Sample FASTQC reports displaying varying metrics:

FASTQC report showing per-base sequence quality, with most bases maintaining high quality across reads and slight drops at the read ends typical of Illumina data.
FASTQC report showing per-base sequence quality, where read quality declines toward the end, indicating potential sequencing degradation or lower confidence in base calls at later positions.
FASTQC report showing a decline in per-base sequence quality toward the end of reads, indicating significant quality drop-off and potential sequencing errors in later positions.

Read More

Previous
Next
RC Logo RC Logo © 2025 The Rector and Visitors of the University of Virginia